We narrowed to 4,276 results for: 424

1. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-SKP2

   Plasmid

   #215092

   Purpose

   Expresses FLAG-SKP2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1410

   Plasmid

   #143267

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RNF2 (RNF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY0663 ACTB atgRNA with v2 scaffold

   Plasmid

   #179108

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY0209 ACTB Nicking guide +48

   Plasmid

   #179109

   Purpose

   ACTB N-term Nicking guide 1 +48 guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term Nicking guide 1 +48 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-SOD2-2A-Catalase-WPRE

   Plasmid

   #67635

   Purpose

   AAV vector expressing both SOD2 and mitochondrial targeted Catalase

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   SOD-2A-Catalase (Sod2, Cat Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Deleted the Peroxisome Targeting Signal from Cata…

   Promoter

   CMV

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1D V5-His-TOPO-KGA.wt

    Plasmid

    #110332

    Purpose

    Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

    Depositor

    Sandra Martha Gomes Dias

    Article

    Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1932

    Plasmid

    #142292

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUN (JUN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-Blast ACSL4

    Plasmid

    #173164

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2052

    Plasmid

    #142911

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2091

    Plasmid

    #144382

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-hATG7CS

    Plasmid

    #87868

    Purpose

    Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 C572S mutant (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C572S mutant

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2053

    Plasmid

    #142912

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF2079

    Plasmid

    #141911

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-GGamma5-GFP2

    Plasmid

    #166777

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma5-GFP2 (GNG5 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-sgCrebbp#2/Cre

    Plasmid

    #193210

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#2 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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