We narrowed to 18,344 results for: multi
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Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralExpressionMammalianPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
UsePlant t-dna plasmidPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCWXPGR-pTF-betaCatenin
Plasmid#114281PurposeTET-inducible expression of a constitutively active form of the BetaCatenin proteinDepositorAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP
Plasmid#32145DepositorPublicationInsertloxP> STOP STOP loxP>
ExpressionInsectAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mRFP-FKBP12-5ptpase domain
Plasmid#67516PurposeRecruitable human type IV 5-phosphatase domain (214-644)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmRFPMutationINPP5E has C641A to destroy the C-terminal CAAX d…Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
delta DE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBPhsFlp1
Plasmid#32148PurposeExpresses FLP recombinase in DrosophilaDepositorPublicationInserthsFlp1
ExpressionInsectMutationAspartic Acid at Amino Acid 5PromoterHsp70Bb promoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HSC1-HS4-GiP
Plasmid#58540PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTRDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
STING-miRFP680
Plasmid#200940PurposeSTING fused with miRFP680 for live cell imagingDepositorInsertsUseLentiviralAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRExpressionBacterialAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYC-Skp-TF
Plasmid#83921PurposeCo-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.DepositorInsertsSkp
Trigger Factor
ExpressionBacterialPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
TagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianPromoterCAG, TetOnAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)
Plasmid#84777PurposeSecond generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neuronsDepositorAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRExpressionMammalianAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTEF2
Plasmid#43971DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterTEFAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits