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zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGS-HDAC1

Plasmid

#109209

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human, Synthetic)

Expression

Insect

Promoter

Polyhedrin

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Publication

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNA500

Plasmid

#149617

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTS-sgRNA500

Plasmid

#149616

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTS-sgRNAflaB

Plasmid

#149624

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNAflaB

Plasmid

#149625

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-CreStopDeleted

Plasmid

#139546

Purpose

Cre ORF without stop codon between attL1-attL2 on Gateway entry vector

Depositor

Minoru Ko

Insert

Cre

Use

Cre/Lox

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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HA-CREST (C89)

Plasmid

#22961

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Tags

HA

Expression

Mammalian

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
pIDS-LSD1-C

Plasmid

#109160

Purpose

Encodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Publication

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)

Expression

Insect

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113609

Purpose

PPI reporter

Depositor

Xiaokun Shu

Publication

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR2

Expression

Mammalian

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-G4DBD-CREST (C91)

Plasmid

#22963

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Tags

G4DBD

Expression

Mammalian

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113612

Purpose

PPI reporter

Depositor

Xiaokun Shu

Publication

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

S1P1

Expression

Mammalian

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

Plasmid

#48307

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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