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non-targeting control gRNA (BRDN0001147780)

Plasmid

#80226

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PI4K2A gRNA (BRDN0001147595)

Plasmid

#76822

Purpose

3rd generation lentiviral gRNA plasmid targeting human PI4K2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PI4K2A (PI4K2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp12

Plasmid

#168969

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp12 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PDPK1 gRNA (BRDN0001146405)

Plasmid

#77453

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Emerald-MannII-N-10

Plasmid

#54150

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MannII (Man2a1 Mouse)

Tags

Emerald

Expression

Mammalian

Mutation

aa 1-112 of MannII aa 1-112 of MannII

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
7432 Bicistronic_GFP_ires_puro

Plasmid

#64336

Purpose

This a retroviral expression plasmid expressing GFP along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

GFP

Use

Retroviral

Tags

GFP

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-KHC-BZ

Plasmid

#160427

Purpose

Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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WEE1 gRNA (BRDN0001149349)

Plasmid

#77367

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WEE1 (WEE1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet2 enhancer H1

Plasmid

#63883

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 enhancer fragment H1

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001146465)

Plasmid

#77174

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001146597)

Plasmid

#77175

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001145078)

Plasmid

#77176

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NADK2 gRNA (BRDN0001487085)

Plasmid

#78093

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MTOR gRNA (BRDN0001147813)

Plasmid

#76783

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MTOR gRNA (BRDN0001145082)

Plasmid

#76782

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCQ gRNA (BRDN0001144886)

Plasmid

#75553

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCQ (PRKCQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCQ gRNA (BRDN0001145693)

Plasmid

#75554

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCQ (PRKCQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-DVL2deltaDIX

Plasmid

#114276

Purpose

TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

DVL2 (Dvl2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

deletion of DIX domain

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

Plasmid

#87917

Purpose

Gateway cloning

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgHTT1, sgGFP2, shCas9

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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PRKD1 gRNA (BRDN0001146537)

Plasmid

#77228

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD1 (PRKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001145869)

Plasmid

#80256

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ROCK1 gRNA (BRDN0001147709)

Plasmid

#77593

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK1 (ROCK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WEE1 gRNA (BRDN0001145570)

Plasmid

#77368

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WEE1 (WEE1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP4

Plasmid

#119876

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PABPN1L

Plasmid

#155807

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PABPN1L (PABPN1L Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PFKFB3 gRNA (BRDN0001146230)

Plasmid

#76462

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB3 (PFKFB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SIK2 gRNA (BRDN0001148975)

Plasmid

#77139

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK2 (SIK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-f1(-)-SEC61B-mEGFP

Plasmid

#115921

Purpose

donor to insert mEGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF'- AmTryoshka1;3-LS-F138I-T78H

Plasmid

#100963

Purpose

Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)

Expression

Yeast

Mutation

F138I, T78H

Promoter

PMA

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1 gRNA (BRDN0001145759)

Plasmid

#76457

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
7749 Bicistronic_ires_hygro

Plasmid

#64374

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GSK3A gRNA (BRDN0001148594)

Plasmid

#76370

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3A (GSK3A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 HCoV-NL63 E_stop

Plasmid

#168684

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223 HCoV-NL63 E_nostop

Plasmid

#168666

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEntry_HCoV-NL63-Envelope

Plasmid

#168924

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Envelope (HCNV63gp4 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OSR1 gRNA (BRDN0001146927)

Plasmid

#76147

Purpose

3rd generation lentiviral gRNA plasmid targeting human OSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

OSR1 (OSR1, OXSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OSR1 gRNA (BRDN0001145611)

Plasmid

#76148

Purpose

3rd generation lentiviral gRNA plasmid targeting human OSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

OSR1 (OSR1, OXSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OSR1 gRNA (BRDN0001145425)

Plasmid

#76149

Purpose

3rd generation lentiviral gRNA plasmid targeting human OSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

OSR1 (OSR1, OXSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6321 - 6360 of 160365 results

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