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pHAGE-NOTCH2NL-Q172_G173delinsHC

Plasmid

#116440

Purpose

Lentiviral expression of NOTCH2NL Q172_G173delinsHC

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

Q172_G173delinsHC

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-R87M

Plasmid

#116441

Purpose

Lentiviral expression of NOTCH2NL R87M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

R87M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-S165F

Plasmid

#116442

Purpose

Lentiviral expression of NOTCH2NL S165F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

S165F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-OXA1L-L57F

Plasmid

#116445

Purpose

Lentiviral expression of OXA1L L57F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

OXA1L (OXA1L Human)

Use

Lentiviral

Mutation

L57F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-OXA1L-P58S

Plasmid

#116446

Purpose

Lentiviral expression of OXA1L P58S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

OXA1L (OXA1L Human)

Use

Lentiviral

Mutation

P58S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MRPS31-R219C

Plasmid

#116437

Purpose

Lentiviral expression of MRPS31 R219C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MRPS31 (MRPS31 Human)

Use

Lentiviral

Mutation

R219C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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mTet1 flox donor

Plasmid

#117147

Purpose

Making Tet1 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAG413GAL-ccdB

Plasmid

#14141

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-ccdB

Plasmid

#14147

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

Plasmid

#191527

Purpose

Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

CRISPR and Synthetic Biology

Tags

mNeonGreen (mNG) + 3xFLAG_TwinStrep

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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spoT_NS1

Plasmid

#100914

Purpose

expresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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spoT-

Plasmid

#100913

Purpose

deletes synpcc7942_1377 (spoT)

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence

Use

Cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_SUZ12:1-739

Plasmid

#211009

Purpose

Mammalian expression of full-length human SUZ12. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SUZ12:M1-L739 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXO3:M1-F471 (FBXO3 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_NUP214:1-2090

Plasmid

#211220

Purpose

Mammalian expression of full-length human NUP214. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NUP214:M1-S2090 (NUP214 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_DCAF12:1-453

Plasmid

#210966

Purpose

Mammalian expression of full-length human DCAF12. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_LLGL1:1-1064

Plasmid

#211214

Purpose

Mammalian expression of full-length human LLGL1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LLGL1:M1-K1064 (LLGL1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_LLGL1:1-1064

Plasmid

#211288

Purpose

Mammalian expression of full-length human LLGL1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LLGL1:M1-K1064 (LLGL1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_ATG16L1:1-607

Plasmid

#211234

Purpose

Mammalian expression of full-length human ATG16L1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L1:M1-Y607 (ATG16L1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

T300A natural variant (VAR_021834)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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