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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLX_317-C7orf26v1

Plasmid

#178537

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-mGSDMD

Plasmid

#111560

Purpose

Express mouse GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-U2AF2-TurboID-HA-rtTA

Plasmid

#226997

Purpose

Integrative plasmid to express U2AF2-TurboID in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Insert

U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)

Tags

TurboID-HA

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-UBF1-C1

Plasmid

#134532

Purpose

Expresses YFP-UBF1 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

UBF1 (UBTF Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MIC60-mCherry

Plasmid

#232789

Purpose

Encodes an mCherry-tagged MIC60/IMMT

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

MICOS complex subunit MIC60 (IMMT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL1

Plasmid

#137759

Purpose

Expression of GABARAPL1

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL1 (GABARAPL1 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRNP70_sgRNA1

Plasmid

#201620

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRNP70 (SNRNP70 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRNP70_sgRNA2

Plasmid

#201621

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRNP70 (SNRNP70 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-Fc-His

Plasmid

#72098

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Arf1-GFP

Plasmid

#39554

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylation factor 1 (ARF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Plenti-ARE-HSVTK-luc

Plasmid

#161789

Purpose

Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

Depositor

Hans-Guido Wendel

Article

Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

Inserts

Luciferase-T2A-HSV-TK
mCherry P2A blasticidin

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a and Murine Gclc ARE

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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LiPOP2

Plasmid

#168266

Purpose

A single-component optogenetic tool for photoswitchable pyroptosis

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

mGSDMD(1-276) (Gsdmd Mouse)

Tags

cpLOV2, mCherry

Expression

Mammalian

Mutation

R138A, K146A, R152A, R154A

Promoter

CMV

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-hESRRB

Plasmid

#60434

Purpose

Over-expression of human ESRRB

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

ESRRB (ESRRB Human)

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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VAMP8-mCherry

Plasmid

#92424

Purpose

Expression of VAMP8 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP8 (Vamp8 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

A36S (Please see Depositor Comments below)

Promoter

CMV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLJY1 DD-FAM98B

Plasmid

#211352

Purpose

Constitutive lentiviral expression of DD-V5-FAM98B fusion protein

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B (FAM98B Human)

Use

Lentiviral

Tags

FKBP12 DD and V5

Promoter

EF1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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