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pcDNA3.1-FLT3-V5/HIS

Plasmid

#201985

Purpose

expression of human FLT3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

FLT3 receptor tyrosine kinase (FLT3 Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3R5

Plasmid

#23819

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R5 (PIK3R5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MAC_C_ROS1

Plasmid

#187766

Purpose

MAC-tagged gene expression of human ROS1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROS1 (ROS1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Promoter

CMVT (Enhanced CMV)

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A2

Plasmid

#132126

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A2 (SLC2A2 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pMX156

Plasmid

#29482

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-beta (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
RFP-KIF1B beta-FL

Plasmid

#79111

Purpose

Mammalian expression of RFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

RFP-KIF1B beta full length (KIF1B Human)

Tags

RFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pLKO-puro-sh-mFAK B

Plasmid

#37018

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

sh-mFAK B (Ptk2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pLV-Kif5b-Myc

Plasmid

#186614

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A7

Plasmid

#132094

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A7 (SLC38A7 Human)

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC10A1

Plasmid

#131907

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A1 (SLC10A1 Human)

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pALPS tat-P2A-rev

Plasmid

#101331

Purpose

Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat and rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEntr_Myc-BirA-G3-ER

Plasmid

#173814

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Use

Gateway shuttle vector

Tags

Myc

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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hum sgk promoter F4R4

Plasmid

#83426

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

Insert

human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)

Use

Luciferase

Tags

Luciferase

Promoter

sgk

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

Suzanne Jackowski, T. J. Kappock

Article

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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IL2 F42A pAcGP67a

Plasmid

#41807

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Tags

6x His

Expression

Insect

Mutation

Phenylalanine 42 to Alanine

Promoter

Polyhedron

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only