We narrowed to 18,031 results for: Mut

1. AID-C12*-dCas9-BE4max

   Plasmid

   #216731

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-dCas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_ATP10B-E993A

   Plasmid

   #203698

   Purpose

   expresses ATP10B with the pathogenic mutation E993A

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_ATP10B-E210A

   Plasmid

   #203697

   Purpose

   expresses ATP10B with the catalytic mutation E210A

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E210A

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3_ATP10B-D433N

   Plasmid

   #203696

   Purpose

   expresses ATP10B with the catalytic mutation D433N

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D433N

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_ATP10B-V748L

   Plasmid

   #203702

   Purpose

   expresses ATP10B with the pathogenic mutation V748L

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V748L

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_ATP10B-I1038T

   Plasmid

   #203700

   Purpose

   expresses ATP10B with the pathogenic mutation I1038T

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I1038T

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_ATP10B-R153X

   Plasmid

   #203701

   Purpose

   expresses ATP10B with the pathogenic mutation R153X

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R153X

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_CMV_dHMGA_mKate2_CLEAVAGE

   Plasmid

   #167337

   Purpose

   Expresses mKate2 fused to TFAM dHMGA mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Truncation mutant containing MTS (1-42) as well a…

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE

   Plasmid

   #167338

   Purpose

   Expresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Truncation mutant containing MTS (1-42) as well a…

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid

    #153440

    Purpose

    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode A knock-out mutant
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 191 and leucine 195 on XDocB were mutate…

    Promoter

    T7 promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C

    Plasmid

    #113730

    Purpose

    Express double mutant RGS7- R44C+V65C in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R44C and V56C

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pb-NES-ZapCV5 (cpV143)

    Plasmid

    #112061

    Purpose

    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV5
    

    Use

    Tags

    Nuclear Export Signal (NES) derived from HIV-1

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    CBA

    Available Since

    July 9, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pLuc-Optop38i-dsm

    Plasmid

    #89748

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDC43-FIT2-FLL[157-159]AAA

    Plasmid

    #96994

    Purpose

    Express GFP-tagged mutated mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Amino acid residues 157-159 were mutated (FLL[157…

    Promoter

    35S

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-CARMA2sh-delta458-500

    Plasmid

    #97074

    Purpose

    Expresses mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 458-500

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid

    #98598

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid

    #98599

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CARD14-L156P

    Plasmid

    #69918

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    L156P

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pIS1 mHK1

    Plasmid

    #60795

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    HK1 3'UTR and mutated miR-155 binding site (HK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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