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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Expression

Mammalian

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Article

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTi4.0_SpdCas9_pmcDA1

Plasmid

#204613

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMT85_SpdCas9_pmcDA1

Plasmid

#204614

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hSURF4-FLAG

Plasmid

#159139

Purpose

Hu SURF4 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

surfeit 4 (SURF4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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EB3-GFP

Plasmid

#190164

Purpose

Expresses EB3-GFP in mammalian cells

Depositor

Anna Akhmanova

Article

Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

Insert

EB3-GFP

Tags

GFP

Expression

Mammalian

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Tags

V5

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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plRL2

Plasmid

#163631

Purpose

Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJV53-Cas12a

Plasmid

#158706

Purpose

CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Promoter

TetO

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer X-null replicon

Plasmid

#65461

Purpose

containing 1.3 units of the X-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

Insert

1.3 units of the X-null HBV genome

Expression

Mammalian

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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