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Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mycRSK2wt
Plasmid#15827DepositorInsertRibosomal S6 kinase 2 (Rps6ka3 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationwild typePromoterAvailable sinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pK7WG2-mitoTAL1-orf125
Plasmid#158736PurposePlant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)DepositorInsertmitoTALEN left and right to target orf125 (orf138)
UseTALENTagsExpressionMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614
Plasmid#158074PurposeExpresses SARS-CoV-2 spike proteinDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.FLAG.VSVg_NGFR
Plasmid#158233PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.FLAG.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
Plasmid#158654Purposemaking transgenics expressing a QUAS driven YFP and NTR 2.0DepositorInsert5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP444
Plasmid#158802PurposePlasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-3
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 WT
Plasmid#158541PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletionPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-EBFP2
Plasmid#158562PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAmotl2 v3
Plasmid#158602Purposelenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2DepositorInsertAmotl2 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
acGFP Peroxisome visual barcode
Plasmid#158674Purposetagging clonal populations with Peroxisomal acGFP fluorescent protein. Adding Peroxisome localization signal to acGFP WCDepositorInsertacGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK2_IDG-K
Plasmid#158193PurposeGateway destination clone of CDK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK2 (CDK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 stuffer v3
Plasmid#158046Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ND4 right TALE–G1397 DddAtox C
Plasmid#158097Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1397 DddAtox N
Plasmid#158094Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only