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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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gTEMP_pcDNA3

Plasmid

#89583

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

gTEMP

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH

Plasmid

#86470

Purpose

Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

Depositor

Akash Gunjan

Article

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Type

Empty backbone

Tags

HIS10-TEV-HA

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFC330

Plasmid

#87842

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
pyrG

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMCS.DT-A

Plasmid

#22730

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

DT-A

Use

Bac recombineering

Mutation

This is a gap-repair vector designed for retrievi…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pVQ CMV NanoV1-2a-EGFP ferritin

Plasmid

#79649

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Article

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanillin 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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BDNF-SEP

Plasmid

#83955

Purpose

BDNF with pH sensitive fluorophore for visualization of release

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Brain derived neurotrophic factor (Bdnf Mouse)

Tags

SEP

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-IL1b

Plasmid

#73953

Purpose

Express murine pro-interleukin-1 beta in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

interleukin-1 beta (Il1b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Sox10

Plasmid

#45843

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pGAMA-YAP

Plasmid

#74942

Purpose

Lentiviral over-expression vector for YAP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

T2A-mCherry

Promoter

EF1α

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-8xHis-Ub

Plasmid

#107392

Purpose

Mammalian expression 8xHis-Ub

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

8 tandem repeats of 6xHis-Ub (See comments below)

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

Plasmid

#162279

Purpose

Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN2A Variant 1, stabilized (SCN2A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp4551.

Promoter

CMV

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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