We narrowed to 8,613 results for: Dos
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Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only
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pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 linker deletion
Plasmid#68806PurposeExpress Galectin-8 linker deletion protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
TagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available SinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_zeo
Plasmid#184913PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_zeo
Plasmid#184917PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180 EACMCV DNA-B 1.6mer
Plasmid#159137PurposeInfectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tip alpha (21-192)
Plasmid#23157DepositorInsertTNF-α-inducing protein
TagsHisExpressionBacterialMutationdeleted amino acids 1-20Available SinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 cyclin B1 L45A (1324)
Plasmid#61851Purposemammalian expression of cyclin B1 L45 A mutant fused to VenusDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tip alpha (28-192)
Plasmid#23158DepositorInsertTNF-α-inducing protein
TagsHisExpressionBacterialMutationdeleted amino acids 1-27Available SinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
palm-Bassoon
Plasmid#250162PurposeEncodes a version of rat Bassoon with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression.DepositorInsertpalmitoylated Bassoon construct (N-terminal 94 aa replaced by palmitoylation consensus) (Bsn Rat)
ExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-deleted
Plasmid#23161DepositorInsertRibonuclease assay sub2
UseIn vitro transcriptionAvailable SinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
OR52-consensus
Plasmid#208080PurposeConsensus OR52 engineered from human OR52 family membersDepositorInsertOR52-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR1-consensus
Plasmid#208059PurposeConsensus OR1 engineered from human OR1 family membersDepositorInsertOR1-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR2-consensus
Plasmid#208060PurposeConsensus OR2 engineered from human OR2 family membersDepositorInsertOR2-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR4-consensus
Plasmid#208061PurposeConsensus OR4 engineered from human OR4 family membersDepositorInsertOR4-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hNR2F2-RFP
Plasmid#22926DepositorInserthuman nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only