We narrowed to 17,761 results for: IGH@;

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KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

Plasmid

#124174

Purpose

piggyBac-based Dox-inducible expression (weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVtight-HNF1A-FLAG-Hygro

Plasmid

#183232

Purpose

Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Use

Lentiviral

Tags

AviTag and FLAG tag

Promoter

tight TRE

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TIGHT-CYCLINF-Tet-OFF

Plasmid

#32977

Depositor

Michele Pagano

Insert

CYCLIN F (CCNF Human)

Use

Lentiviral

Tags

Flag and mCherry

Expression

Mammalian

Promoter

P-TIGHT

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD(V28R)

Plasmid

#212955

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C-wildtype

Plasmid

#179687

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-GFP (467)

Plasmid

#65491

Purpose

negative control for expression assays

Depositor

Roderic Guigo, Rory Johnson

Insert

EGFP

Use

Luciferase

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTre-tight-SV40-luciferase (496)

Plasmid

#65489

Purpose

Inducible expression of coding/noncoding genes

Depositor

Roderic Guigo, Rory Johnson

Insert

SV40-luciferase

Use

Luciferase

Expression

Mammalian

Promoter

tight Tre promoter

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-ND1 Right-GSVG-UGI

Plasmid

#187413

Purpose

human mitochondrial DNA ND1 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Promoter

None

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-FLAG-CYLD-C601A

Plasmid

#60029

Purpose

CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

TRE

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
ND4 right TALE–G1333 DddAtox N

Plasmid

#158096

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-ND4 Right-GSVG-UGI

Plasmid

#187414

Purpose

human mitochondrial DNA ND4 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

AAV and TALEN

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ND4 Right-E1347A-UGI

Plasmid

#187415

Purpose

human mitochondrial DNA ND4 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

AAV and TALEN

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-Hottip (522)

Plasmid

#65490

Purpose

positive control for lncRNA expression assay

Depositor

Roderic Guigo, Rory Johnson

Insert

Hottip (HOTTIP Human)

Use

Luciferase

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Nonsense-Right-G1333-C

Plasmid

#169215

Purpose

Express DdCBE for ND5 Nonsense Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Expression

Mammalian

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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KA2880_pPB-TREtight-FLAG-Esrrb-InO

Plasmid

#124178

Purpose

piggyBac-based Dox-inducible expression of FLAG-Esrrb

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA2944_pPB-TREtight-Esrrb-FLAG-InO

Plasmid

#124179

Purpose

piggyBac-based Dox-inducible expression of Esrrb-FLAG

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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