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Plasmid#68162PurposeProvides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid partDepositorInsertAt2S3 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_mScarlet
Plasmid#188039PurposeExpresses CapChR2-mScarlet in mammalian neuronsDepositorInsertCapChR2-mScarlet
UseAAVTagsmScarletExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-D156N-T159C 2A tDimer
Plasmid#52494PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, D156N, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR1-FusionRed
Plasmid#105679PurposeExpresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoterDepositorInsertstGtACR1
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR1-FusionRed
Plasmid#105678PurposeExpresses a soma-targeted GtACR1 in Cre-expressing neuronsDepositorHas ServiceAAV5InsertstGtACR1
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianMutationPromoterhSyn1Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 YFP-CaRhAC
Plasmid#101721PurposeYellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracelluDepositorInsertYFP CaRhAC
UseAAVTagsYFPExpressionMammalianMutationPromoterhSyn1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 Mustard Weed, CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 Mustard Weed, CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-C-GRF4-P2A:mTagBFP2
Plasmid#216140PurposeControls for Bicistronic BiFCDepositorInsertGRF4 isoform 1
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-N-MPK8-P2A:mCherry
Plasmid#216138PurposeControls for Bicistronic BiFCDepositorInsertMPK8
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT10446
Plasmid#192353PurposeL0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stopDepositorInsertTTCG_Stop-t35S_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT9403
Plasmid#192352PurposeL0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stopDepositorInsertTTCG_Stop-tNOS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TaraACR3-pmCherry-C1
Plasmid#204962PurposeExpression of TaraACR3 in fusion with mCherry in mammalian cellsDepositorInsertTaraACR3
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only