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We narrowed to 25,151 results for: crispr

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Showing: 301 - 320 of 25151 results

  1. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86556-plasmid-map-sequence-id-176429

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  5. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139988-plasmid-map-sequence-id-275338

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  8. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit
    #1000000079
    Purpose
    Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.
    Depositor
    Thoru Pederson
    Application
    Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  12. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Promoter
    T7
    Available Since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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  19. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid Kit
    #1000000159
    Purpose
    Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots.
    Depositor
    Vladimir Nekrasov
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 320 of 25151 results