We narrowed to 166,561 results for: Gene

1. pCAG Cas9-2A-Citrine

   Plasmid

   #92358

   Purpose

   CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDC32B-AtMIR390a-B/c

   Plasmid

   #51776

   Purpose

   Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Plant expression

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Htr5b(3.7)-tTA-sv40

   Plasmid

   #184412

   Purpose

   AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Htr5b gene promoter-tTA
   

   Use

   AAV

   Tags

   tTA

   Expression

   Mammalian

   Promoter

   Mouse Htr5b gene

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-P29

   Plasmid

   #120040

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-AtMIR390a-B/c

   Plasmid

   #51778

   Purpose

   Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Gateway entry vector

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRvTUB-mEGFP-NLS

   Plasmid

   #205011

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPEPY-PF6-lacI

   Plasmid

   #85589

   Purpose

   Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

   Insert

   lacI
   

   Expression

   Bacterial

   Promoter

   PF6

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Promoter

   pPEBV

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRvSAHS1-mEGFP-NLS

    Plasmid

    #205005

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRvCAHS3-mEGFP-NLS

    Plasmid

    #205004

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Equalizer-L plasmid

    Plasmid

    #169732

    Purpose

    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164041

    Purpose

    CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLC-242

    Plasmid

    #73189

    Purpose

    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    

    Expression

    Bacterial

    Promoter

    chloramphenicol promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330a dCas9-VP64

    Plasmid

    #92363

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-Z29

    Plasmid

    #120072

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB2-neo-TET

    Plasmid

    #51287

    Purpose

    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    sine B2
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    enhancer from 7SL gene

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMDC123SB-AtMIR390a-B/c

    Plasmid

    #51775

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Equalizer-H plasmid

    Plasmid

    #169367

    Purpose

    Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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