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SP65U1-301a
Plasmid#105821Purposeplasmid for the overexpression of miR-301a under the U1 expression cassette inserted in the pSP65 plasmidDepositorInsertMIRNA301 (Mir301 Mouse)
UseTagsExpressionMammalianMutationPromoterU1Available sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro
Plasmid#214680PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHflu1
Plasmid#203878PurposepSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transferDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
cTRE-GFP-miR30_shRen
Plasmid#135666PurposeIntroduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shRenilla into (ES) cells by recombination-mediated cassette exchangeDepositorInsertshRenilla
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-CRE-T2A-BleoR
Plasmid#172300PurposeIn vitro transcription of CRE-T2A-BleoR synthetic construct for the excision of floxed cassettesDepositorInsertCRE-T2A-BleoR
UseCre/LoxTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP
Plasmid#174893Purposeamber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassetteDepositorInsertmCherry and EGFP
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB Neo* PB
Plasmid#138413PurposeMinimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; PiggybacTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
G-RCAS (A)
Plasmid#15182DepositorInsertGateway cassette
UseRNAi and Retroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDTA-5
Plasmid#128845PurposeGateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy numberDepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1CH blast
Plasmid#69612PurposeExpresses a blasticidin tagged codon optimized L1PA1DepositorInsertL1PA1
UseTagsblast cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBS-L1PA13ACHmneo
Plasmid#69613PurposeExpresses a neomycin tagged codon optimized L1PA13ADepositorInsertL1PA13A
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-pegRNA-ST2-com-vector
Plasmid#136272PurposeExpresses PE2 and ST2-com modified pegRNA cassetteDepositorInsertPE2, pegRNA scaffold
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
cTRE-GFP-miR30_shPten.1523
Plasmid#135667PurposeIntroduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchangeDepositorInsertshPten
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pREP4xV5_ccdB
Plasmid#79016PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP3xV5_ccdB
Plasmid#79015PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG7084 pG46-46
Plasmid#232621PurposepG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).DepositorTypeEmpty backboneUsePrecursor plasmid to generate modified dna circlesTagsExpressionMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA
Plasmid#233031PurposeTo express a DjCas13d-compatible gRNADepositorInsertDjCas13d-compatible gRNA expression cassette
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:MCS+:NosT
Plasmid#212160PurposeThis binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_GFP-mCherry_ChInt++
Plasmid#187393Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_GFP-mCherry_ChInt--
Plasmid#187394Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_mCherry-GFP_ChInt--
Plasmid#187396Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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