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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,220 results for: ATO

Showing: 3221 - 3240 of 20220 results

  1. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

    Plasmid
    #171663
    Purpose
    QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171663-plasmid-map-sequence-id-339028

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  9. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    155378-plasmid-map-sequence-id-324490

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  10. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

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  11. pGBW-m4046846

    Plasmid
    #145575
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145575-plasmid-map-sequence-id-287752

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  12. MTK6_013

    Plasmid
    #123867
    Purpose
    Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    WPRE-Blast (no terminator)
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123867-plasmid-map-sequence-id-249818

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  17. pHL2073

    Plasmid
    #62837
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::glmS
    Expression
    Bacterial
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pHL2075

    Plasmid
    #62838
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    glmS
    Expression
    Bacterial
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pHL2085

    Plasmid
    #62840
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    glmS
    Expression
    Bacterial
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pHL2084

    Plasmid
    #62839
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::glmS
    Expression
    Bacterial
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3221 - 3240 of 20220 results