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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,299 results for: sgRNA

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Showing: 3221 - 3240 of 17299 results

  2. pKLV-XAF1

    Plasmid
    #140290
    Purpose
    To knockout murine XAF1 gene by CRISPR-Cas9 system
    Depositor
    Adriano Aguzzi
    Insert
    XAF1 sgRNA (Xaf1 Mouse)
    Use
    Lentiviral
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    140290-plasmid-map-sequence-id-337087

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  7. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  9. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid
    #159283
    Purpose
    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Human INS sgRNA
    Human INS guide RNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159283-plasmid-map-sequence-id-311907

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  17. pX459-sgRNA3-RPB1-N-term

    Plasmid
    #195112
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195112-plasmid-map-sequence-id-383882

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  18. pX459-sgRNA1-RPB1-N-term

    Plasmid
    #195110
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195110-plasmid-map-sequence-id-383880

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  19. pX459-sgRNA2-2-RPB1-C-term

    Plasmid
    #195109
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against last exon of RPB1 (C-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195109-plasmid-map-sequence-id-383879

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  20. pX330 _CRISPIE donor B9

    Plasmid
    #172850
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172850-plasmid-map-sequence-id-341005

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Showing: 3221 - 3240 of 17299 results