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pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGDP1 fosA

Plasmid

#112889

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

fosA

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid EDEN15

Plasmid

#107252

Purpose

EDEN15 RNA motif

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

EDEN15 motif

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mVenus

Expression

Bacterial and Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Article

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF1

Plasmid

#117156

Purpose

Target site TTGCTCTTAGCATCTCGGCTG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF3

Plasmid

#101336

Purpose

target site ATCAGATCTACAATGAAGGGC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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p3.1-NEFH

Plasmid

#26978

Depositor

David Sidransky

Article

Kim et al PLoS One. 2010 . 5(2):e9003.

Insert

NEFH (NEFH Human)

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-IRF9

Plasmid

#117159

Purpose

Target site AATTATCACAAAGAGGACAGG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR

Plasmid

#112175

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF5

Plasmid

#117157

Purpose

Target site TATTTCCCTGTCTCCTTGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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