We narrowed to 7,609 results for: trac
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Plasmid#175278PurposeAAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomatoDepositorInsertNS1; dTomato (POLY Yellow fever virus strain 17D)
UseAAVTagsdTomato (from bidirectional promoter)Promoterbidirectional TRE promoterAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM-Venus
Plasmid#164785PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Gaussia princeps)
TagsGLuc and mVenusExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4-no linker
Plasmid#176925Purposeexpress Granulin 4 without linker 5 in mammalian cellsDepositorInsertGranulin 4 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-5LTR-GW-A
Plasmid#138480PurposeFor expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.DepositorInsertGateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Zdk1-EB1C
Plasmid#107695PurposeC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
TagsZdk1 and mCherryExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TdT
Plasmid#126450PurposeExpresses human TdT in mammalian cells. (pTBL206)DepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-hGTB
Plasmid#26196DepositorInsertTet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusMutationF2A has 2A cleavage site deleted, producing htTA-…Available SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-Necl5-ScNeo
Plasmid#209901PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
TagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
TagsLckExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-Anillin shRNA
Plasmid#187270PurposeLentiviral expression of shRNA targeting AnillinDepositorAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
Tags3x FLAGExpressionInsectPromoterUbi-p63eAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GAP(ARHGAP29)
Plasmid#222632PurposeRho inactivation using rapamycinDepositorInsertmCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
TagsmCherry-FKBPExpressionMammalianPromoterCAGAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
Tags3xNLSExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPBbsr2-Necl-5-ScNeo
Plasmid#170283PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInserta recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Mouse, Synthetic)
TagsmScarlet and mNeonGreenExpressionMammalianAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK4-EE
Plasmid#47577PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4DepositorInsertMKK4-EE (MAP2K4 Human)
Tags3xHA and GSTExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…Available SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only