265,097 results

1. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas12f-GE ver4.1

   Plasmid

   #176544

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.1
   Cas12f-GE ver4.1
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-sRGN3.1-BlastR

   Plasmid

   #220964

   Purpose

   Expresses human codon-optimized sRGN3.1

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   sRGN3.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene

   Pooled Library

   #83971

   Purpose

   Human CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-Neo-Bam p53 wt

   Plasmid

   #16434

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Neo-Bam p53 wt

   Plasmid

   #16434

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Orai1 CFP

   Plasmid

   #19757

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. Orai1 CFP

   Plasmid

   #19757

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161926

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    rTetR(SE-G72P)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Green cGull

    Plasmid

    #86867

    Purpose

    Green fluorescent probe for cGMP

    Depositor

    Tetsuya Kitaguchi

    Article

    Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

    Insert

    Green cGull
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Green cGull

    Plasmid

    #86867

    Purpose

    Green fluorescent probe for cGMP

    Depositor

    Tetsuya Kitaguchi

    Article

    Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

    Insert

    Green cGull
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep

    #100845-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

20. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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