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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 41,246 results for: Eras

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Showing: 3241 - 3260 of 41246 results

  6. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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  13. pACT2-μ1A

    Plasmid
    #198168
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198168-plasmid-map-sequence-id-387525

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  16. ST*-GFP

    Plasmid
    #163667
    Purpose
    Expresses partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163667-plasmid-map-sequence-id-322800

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  17. psiCHECK2 3'UTR GAC

    Plasmid
    #110401
    Purpose
    Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110401-plasmid-map-sequence-id-227284

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  18. pGEX-2T-RNAPII-A2-14-HA

    Plasmid
    #160689
    Purpose
    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
    Depositor
    Jeffry Corden
    Article
    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.
    Insert
    RNA polymerase II CTD
    Expression
    Bacterial
    Mutation
    Contains an S-to-A mutation in position 2 of 14 o…
    Available Since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    160689-plasmid-map-sequence-id-317780

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Showing: 3241 - 3260 of 41246 results