Addgene

We narrowed to 48,841 results for: des.1

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Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Use

Tags

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-976aa)

Plasmid

#139689

Purpose

Expresses N-teminal Flag tagged aa 1-976 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #1

Plasmid

#179454

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-501aa-YFP

Plasmid

#29601

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-501aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-501

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-373aa-YFP

Plasmid

#29598

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-373

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Promoter

Available since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-413aa-YFP

Plasmid

#29599

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-413

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pBABE (hygro) hYAP 1-1delta

Plasmid

#124150

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-453aa-YFP

Plasmid

#29600

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-453aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-453

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pMT2 Alpha2 delta-1 V6

Plasmid

#89894

Purpose

Mammalian expression of Alpha2 delta-1 V6

Depositor

Annette Dolphin

Article

Kadurin et al Elife. 2016 Oct 26;5. pii: e21143. doi:

Insert

Cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Amino acids L943V, E944V, A945V, E947V, M948V are…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-270aa-YFP

Plasmid

#29597

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-270aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-270

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA8-Flag-LARS(1-360aa)

Plasmid

#139668

Purpose

Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

Leucyl-tRNA Synthetase (LARS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_mCherry-ATG13 HORMA (1-191aa)

Plasmid

#223759

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 HORMA (ATG13 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-191 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST

Plasmid

#223744

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got1 shRNA#1

Plasmid

#220592

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got1 shRNA (Got1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Repo-Man-1-890

Plasmid

#184272

Purpose

Expresses C-terminally truncated GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

truncated Repo-Man after aminoacid 893

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPERFECT-1

Plasmid

#215575

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT-1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT-1 (HOXD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS15+1

Plasmid

#215604

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS15+1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15+1 (CEBPA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 RING

Plasmid

#185353

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 cytoplasmic domain (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Stop codon after aa 393

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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