We narrowed to 15,788 results for: LEA

1. pCMV-BE2

   Plasmid

   #73020

   Purpose

   Expresses BE2 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgOpti

   Plasmid

   #85681

   Purpose

   Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

   Depositor

   Eric Lander, David Sabatini

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1-CMV-DAAO-NES

   Plasmid

   #141107

   Purpose

   Expresses DAAO with nuclear export signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Article

   Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

   Insert

   DAAO-NES
   

   Use

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL0M-S-mTurquoise2-EC18152

   Plasmid

   #137074

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tailless VinculinTS

   Plasmid

   #26020

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   tailless vinculinTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Membrane mCherry

   Plasmid

   #210470

   Purpose

   Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane mCherry (LCK Human, A.victoria, Synthetic)
   

   Use

   Synthetic Biology; Sea urchin, mammal, zebrafish

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL0M-S-mKOκ-EC18154

   Plasmid

   #137077

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

   Plasmid

   #163727

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding three amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.3TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,N135TAG,Y151TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-GRAB_r5-HT1.0

   Plasmid

   #208713

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCN-EF2132tet

    Plasmid

    #107191

    Purpose

    Expresses EF2132 recombinase for performing recombineering in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    EF2132
    tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p23

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-PhoCl1-6His

    Plasmid

    #164033

    Purpose

    Expressed PhoCl1 in a pET expression vector

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl1
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvol-NnSULT1C1-cysDNCQ

    Plasmid

    #188983

    Purpose

    biosynthesize sulfotyrosine in E. coli.

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Inserts

    NnSULT1C1
    cysD
    cysN
    cysC
    cysQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-RSV-tdTomato-2A-GCaMP6s

    Plasmid

    #80316

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Inserts

    tdTomato
    GCaMP6s
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    RSV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbS8c_ddCpf1_final

    Plasmid

    #212040

    Purpose

    Bacterial expression of the CRISPRi system for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    ddCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    L-arabinose-inducible promoter PBAD

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE9-His-p97(wt)

    Plasmid

    #14666

    Depositor

    Graham Warren

    Article

    Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

    Insert

    p97 (Vcp Mouse)
    

    Use

    Tags

    RGS6xHis

    Expression

    Bacterial

    Mutation

    See depositor comments below.

    Promoter

    Available since

    April 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. mini-donor - NKX6.1-nEGFP

    Plasmid

    #206047

    Purpose

    this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-NES-DEVD-mCardinal-NLS

    Plasmid

    #164052

    Purpose

    Fluorescent reporter for caspase-3

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-mCardinal-NLS
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.23-MYC

    Plasmid

    #86461

    Purpose

    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MYC

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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