We narrowed to 15,991 results for: LEA

1. pACS129

   Plasmid

   #204594

   Purpose

   Histone H3.1-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 (H3C3 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1-V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-dCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201152

   Purpose

   Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   dCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: TACGTTCTCTATCACTGAT

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SL2 GFP H2B

   Plasmid

   #227717

   Purpose

   Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   SL2 GFP H2B (nlp-51 Nematode)
   

   Use

   Cloning vector

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT378_pattB-pCA-GFP-pA-attB

   Plasmid

   #52554

   Purpose

   for PhiC31 integrase-mediated cassette exchange

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Expression

   Mammalian

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-RPS6 (pDD1902)

   Plasmid

   #52913

   Purpose

   contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

   Depositor

   Dirk Dittmer

   Article

   Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

   Insert

   HAS6 (RPS6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCN-EF2132tet

   Plasmid

   #107191

   Purpose

   Expresses EF2132 recombinase for performing recombineering in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   EF2132
   tetR
   

   Expression

   Bacterial

   Promoter

   p23

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-NLGN3 R451C

   Plasmid

   #59319

   Purpose

   Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN3 R451C (NLGN3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 451 to Cysteine (R451C mutation)

   Promoter

   CAG

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-RSV-tdTomato-2A-GCaMP6s

   Plasmid

   #80316

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Darrell Kotton

   Article

   Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

   Inserts

   tdTomato
   GCaMP6s
   

   Use

   Lentiviral

   Promoter

   RSV

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-cell-mCer

    Plasmid

    #185601

    Purpose

    Lentiviral construct for expression of mCerulean

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-HA.Tetherin

    Plasmid

    #41068

    Depositor

    Paul Spearman

    Article

    Ali et al PLoS One. 2010 Feb 2;5(2):e9005.

    Insert

    Tetherin (BST2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC1-CMV-DAAO-NES

    Plasmid

    #141107

    Purpose

    Expresses DAAO with nuclear export signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Article

    Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

    Insert

    DAAO-NES
    

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-RalGDS

    Plasmid

    #118315

    Purpose

    Expression of human RalGDS

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RalGDS (RALGDS Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment of human RalGDS 203aa

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEvol-NnSULT1C1-cysDNCQ

    Plasmid

    #188983

    Purpose

    biosynthesize sulfotyrosine in E. coli.

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Inserts

    NnSULT1C1
    cysD
    cysN
    cysC
    cysQ
    

    Expression

    Bacterial

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSSM151: pU6-NlovFz2_omegaRNA-entry

    Plasmid

    #205263

    Purpose

    Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SAFB1-EGFP

    Plasmid

    #32746

    Depositor

    Steffi Oesterreich

    Article

    Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

    Insert

    SAFB1 (SAFB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-RSV-tdTomato-2A-GCaMP6f

    Plasmid

    #80317

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Inserts

    tdTomato
    GCaMP6f
    

    Use

    Lentiviral

    Promoter

    RSV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Membrane mCherry

    Plasmid

    #210470

    Purpose

    Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane mCherry (LCK Human, Synthetic, A.victoria)
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCherry

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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