We narrowed to 26,884 results for: STI

1. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCENPBR pc861

   Plasmid

   #167569

   Purpose

   Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   Homo sapiens centromere protein B (CENPB Human)
   

   Use

   Tags

   Ds-Red

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcmv3-IRG1-H277Y-HA

   Plasmid

   #198185

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The histidine in IRG1 at position 277 mutates to …

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMeCP2miRFP670 pc3942

   Plasmid

   #167568

   Purpose

   The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   MeCp2 (Mecp2 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShuttle.CC9

   Plasmid

   #192931

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA251_ Ptrc.x.tetO2::D-HicDH

   Plasmid

   #185456

   Purpose

   CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   D-HicDH from Lactobacillus paracasei DSM 20008
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB’ CsoS2 ΔNTD

   Plasmid

   #162705

   Purpose

   Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deletion of residues 2-256 of CsoS2, Y131D

   Promoter

   PLtet0-1 promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCM’ CbbQ-

   Plasmid

   #162711

   Purpose

   Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Inactivation of CbbQ subunit of CbbOQ rubisco act…

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCM’ ΔacRAF

   Plasmid

   #162710

   Purpose

   Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pCCM' with a deletion of putative rubisco ch…

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Met133I, M1381I, M186I ELL2

    Plasmid

    #127263

    Purpose

    For in vitro translation of human ELL2 with Met133I, M1381I, M186I

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Three Mets (133, 138, 186) to Ileu

    Promoter

    T7

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 563aa ELL2 pEF4B

    Plasmid

    #127273

    Purpose

    Expresses 563aa human WT ELL2

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 truncated at SphI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains 564 N-terminal amino acids ELL2, termina…

    Promoter

    pEF1a

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA tagged Sph ELL2

    Plasmid

    #127270

    Purpose

    For in vitro translation of human WT ELL2 with internal HA tag

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    WT Mets and HA tag at SphI site (8th Met)

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA tagged mid ELL2

    Plasmid

    #127274

    Purpose

    Expresses human ELL2 with HA tag in the middle that does not disrupt function

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with HA at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA inserted at XbaI after Met 186

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Met138ILeu ELL2

    Plasmid

    #127260

    Purpose

    For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met138 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Met186ILeu ELL2

    Plasmid

    #127261

    Purpose

    For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met186 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met186 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Met138ILeu, Met186ILeu ELL2

    Plasmid

    #127265

    Purpose

    For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Two Mets (138,186) to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Met133ILeu ELL2

    Plasmid

    #127259

    Purpose

    For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met133 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Met133ILeu, Met186ILeu ELL2

    Plasmid

    #127264

    Purpose

    For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Two Mets (133,186 )to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Met331Leu ELL2

    Plasmid

    #127262

    Purpose

    For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met331 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met331 to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. COOHend ELL2

    Plasmid

    #127255

    Purpose

    Expresses the C terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (C-terminal end) (ELL2 Human)
    

    Use

    Other

    Tags

    Expression

    Mammalian

    Mutation

    contains only the last 389 aa

    Promoter

    pEF1a

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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