We narrowed to 22,588 results for: his

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pLZL2386

Plasmid

#48682

Purpose

pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

Depositor

Suzanne Sandmeyer

Article

Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

Insert

PFV Integrase

Tags

His

Expression

Bacterial

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pET PPL His6 LIC cloning vector (2J-T)

Plasmid

#37182

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and PPL

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_6xH

Plasmid

#186461

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-MCM3/5

Plasmid

#116950

Purpose

Expresses human MCM3 and human MCM5

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM5 (MCM5 Human)
human MCM3 (MCM3 Human)

Tags

His6

Expression

Bacterial

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-mGSDMD

Plasmid

#111560

Purpose

Express mouse GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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eSrtA(4S-9) pET29b

Plasmid

#75146

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

N98D S102C A104V A118T F122A K134R F144L I182V E1…

Promoter

T7

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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wt HIV-1 integrase in pET15b

Plasmid

#61668

Purpose

wild type HIV-1 integrase in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

wild type HIV-1 integrase

Tags

His

Promoter

T7 promotor

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
mPapaya-pBAD

Plasmid

#54838

Purpose

Localization: Protein Expression Vector , Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

6xHis and mPapaya

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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Nano-lantern/pRSETB

Plasmid

#51969

Purpose

bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRAM Flag

Plasmid

#41551

Depositor

Kate Fitzgerald, Doug Golenbock

Article

Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

Insert

TRAM (TICAM2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-ec_bPAC

Plasmid

#28135

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

E.coli adapted photoactivated adenylyl cyclase

Tags

His

Expression

Bacterial

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only