We narrowed to 10,401 results for: 158

1. pBIG1e-CDK13/Cyclin K

   Plasmid

   #231731

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK13 (CDK13 Human)
   CCNK (CCNK Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-UAS-zArchon1-KGC-EGFP-ER2

   Plasmid

   #108427

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zArchon1-KGC-EGFP-ER2
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_mEGFP-TAZ-CAMTA1-KS

   Plasmid

   #237677

   Purpose

   For overexpression of mEGFP-TAZ-CAMTA1-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TAZ-CAMTA1-KS (WWTR1, CAMTA1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CL20_mEGFP_CBFA2T3_GLIS2

   Plasmid

   #205786

   Purpose

   Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CL20_mEGFP_PIEZO1_CBFA2T3

   Plasmid

   #205895

   Purpose

   Express mEGFP-tagged fusion protein, PIEZO1_CBFA2T3 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PIEZO1_CBFA2T3 (PIEZO1, CBFA2T3 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO-GAlphaz-RLuc8

   Plasmid

   #140978

   Purpose

   Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaz-Rluc8 (GNAZ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAB1923 REPAIR.t1-S

   Plasmid

   #176321

   Purpose

   CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt1 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   E488Q/E620G/Q696L, only the deaminase domain (aa …

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Syn-miRFP

   Plasmid

   #108416

   Purpose

   Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-Archon1-KGC-EGFP-ER2

   Plasmid

   #108420

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-2dV-Camui (T305D/T306D)

    Plasmid

    #220368

    Purpose

    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed both Threonine 305 and 306 to aspartic ac…

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB1676 REPAIR.t1

    Plasmid

    #176319

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD649-HAsp-KIR2DL2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156669

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL2 (KIR2DL2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-KIR2DL3-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156670

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL3 (KIR2DL3 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid

    #140974

    Purpose

    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai2-Rluc8 (GNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

    Plasmid

    #108422

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV5

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191163

    Purpose

    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CL20_mEGFP_BRD9_CEP72

    Plasmid

    #205782

    Purpose

    Express mEGFP-tagged fusion protein, BRD9_CEP72 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    BRD9_CEP72 (BRD9, CEP72 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid

    #114069

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid

    #114086

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

    Plasmid

    #181850

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmiRFP-alpha-Tubulin-19-C

    Plasmid

    #108413

    Purpose

    Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    alpha-tubulin b (Tuba1b Mouse)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

    Plasmid

    #114095

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (E174R/S542R/K607R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R, S542R and K607R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTT3-ecdICAM1-ST3-His

    Plasmid

    #210666

    Purpose

    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Synthetic, Human)
    

    Use

    Tags

    Histag and Spytag003

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmiRFP-Vimentin-17-C

    Plasmid

    #108414

    Purpose

    Vimentin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid

    #140977

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoB-Rluc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pD649-HAsp-ICAM1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156868

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ICAM1 (ICAM1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge