We narrowed to 7,594 results for: Lif

1. pMRXIP-GFP-9K0Q

   Plasmid

   #221739

   Purpose

   Stable expression of GFP-9K0Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

   Plasmid

   #196196

   Purpose

   homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
   

   Use

   Homology repair donor

   Tags

   V5-APEX2-mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-FOXL2-cterm

   Plasmid

   #192893

   Purpose

   Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2 C-term sgRNA (FOXL2 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-FGFR2c-V5/HIS

   Plasmid

   #201107

   Purpose

   expression of human FGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-Pan-QKI [N147/6.1R]

   Plasmid

   #114521

   Purpose

   Mammalian Expression Plasmid of anti-Pan-QKI (Human). Derived from hybridoma N147/6.1.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Pan-QKI (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SspB-HaloTag-ITSN1-DHPH

   Plasmid

   #176128

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-O-FUW-ZEB2-P2A-mCherry

   Plasmid

   #203857

   Purpose

   Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTag-NLS-eGFP-SV40

   Plasmid

   #117811

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CSF1R-V5/HIS

    Plasmid

    #201984

    Purpose

    expression of human CSF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    CSF1R receptor tyrosine kinase (CSF1R Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-FOXL2

    Plasmid

    #192914

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 (FOXL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HC20 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135220

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINDUCER20-Tau

    Plasmid

    #92201

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for Tau

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-DDR1-V5/HIS

    Plasmid

    #201100

    Purpose

    expression of human DDR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid

    #205618

    Purpose

    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    T28M, S455R- Please see depositor comment

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid

    #196185

    Purpose

    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Inserts

    GSK3B (GSK3B Human)
    λPPase
    

    Use

    Tags

    GST, HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-MYC

    Plasmid

    #192899

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MYC

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF5-FRT-V5-DEST-GFP-Kif26b

    Plasmid

    #102862

    Purpose

    Expresses GFP-Kif26b in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AC50 pEGFPN1 anti-AMIGO-1 nanobody

    Plasmid

    #135225

    Purpose

    Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-cT3G-cERP2-FOS

    Plasmid

    #192924

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOS

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOS (FOS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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