We narrowed to 8,345 results for: construct

1. pGBW-m4095419

   Plasmid

   #149526

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   d1-1088 d1203-1945

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046950

   Plasmid

   #149258

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046856

   Plasmid

   #145698

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pNH078

   Plasmid

   #42164

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046831

   Plasmid

   #145671

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDS66

   Plasmid

   #48364

   Purpose

   3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
   

   Use

   Cre/Lox; Knockout in t. brucei

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNI2

   Plasmid

   #30997

   Depositor

   Daniel Voytas, Adam Bogdanove

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   repeat NI2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-YFP-HA no-pA

   Plasmid

   #82590

   Purpose

   3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   YFP-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMST665_BB3_L 23_gRNAempty_cas9_BbsI

   Plasmid

   #90278

   Purpose

   BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   Cas9
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHD4

    Plasmid

    #30977

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat HD4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDS67

    Plasmid

    #48365

    Purpose

    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046906

    Plasmid

    #149248

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046797

    Plasmid

    #145676

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pHD7

    Plasmid

    #30980

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat HD7
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046971

    Plasmid

    #145664

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046755

    Plasmid

    #145573

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. dFP

    Plasmid

    #120542

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein (dFP)
    

    Use

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046729

    Plasmid

    #145653

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046859

    Plasmid

    #145626

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046905

    Plasmid

    #145762

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge