We narrowed to 8,661 results for: pcas

1. pCAGGS-MTS(S6A)-EGFP-V5

   Plasmid

   #200817

   Purpose

   expresses MTS(S6A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   Changed Serine 6 to Alanine in MTS

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-MTS(S12A)-EGFP-V5

   Plasmid

   #200818

   Purpose

   expresses MTS(S12A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S12A in MTS

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_mCherry-ATG13 HORMA (1-191aa)

   Plasmid

   #223759

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 HORMA (ATG13 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa 1-191 only

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG/VPS15(R36D)-TSF

   Plasmid

   #232913

   Purpose

   Mammalian Expression

   Depositor

   James Hurley

   Insert

   VPS15 (PIK3R4 Human)
   

   Expression

   Mammalian

   Mutation

   R36D

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/VPS34(W885D/Y884D)

   Plasmid

   #232918

   Purpose

   Mammalian Expression

   Depositor

   James Hurley

   Insert

   VPS34 (PIK3C3 Human)
   

   Expression

   Mammalian

   Mutation

   W885D/Y884D

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-U6-MYC-Guide-hSpCas9

   Plasmid

   #228574

   Purpose

   Expression of human MYC gRNA; CRISPR mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   MYC gRNA (MYC Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 IST1 (322-366)

   Plasmid

   #193038

   Purpose

   Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 322-366

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG CAPN7 V18D-OSF

   Plasmid

   #193042

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   V18D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG CAPN7 L61D-OSF

   Plasmid

   #193043

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   L61D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG CAPN7 F98D-OSF

    Plasmid

    #193044

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    F98D

    Promoter

    Chicken Beta-actin

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CHMP1B 143-199

    Plasmid

    #108255

    Purpose

    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1B residues 143-199 (CHMP1B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 CHMP4A 153-222

    Plasmid

    #108256

    Purpose

    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4A residues 153-222 (CHMP4A Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 CHMP4C 156-233

    Plasmid

    #108257

    Purpose

    Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C residues 156-233 (CHMP4C Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 CHMP5 159-222

    Plasmid

    #108259

    Purpose

    Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP5 residues 148-219 (CHMP5 Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CHMP2B 141-213

    Plasmid

    #104595

    Purpose

    Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2B residues 141-213 (CHMP2B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 CHMP4B 156-224

    Plasmid

    #104596

    Purpose

    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B residues 156-224 (CHMP4B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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