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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 3321 - 3340 of 14582 results

  10. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  11. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  12. pSEMS-SDHB-Halo7Tag

    Plasmid
    #108973
    Purpose
    Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)
    Depositor
    Karin Busch
    Publication
    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
    Tags
    Halo7-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    108973-plasmid-map-sequence-id-213888

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  19. p14-9VN

    Plasmid
    #50930
    Purpose
    Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50930-plasmid-map-sequence-id-422738

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Showing: 3321 - 3340 of 14582 results