We narrowed to 9,799 results for: otos

1. pST_CAHS3_HYPDU (pBS0333)

   Plasmid

   #185179

   Purpose

   For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS3_HYPDU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST_APOE_HUMAN_E114I (pBS0839)

   Plasmid

   #185274

   Purpose

   For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   APOE_HUMAN_E114I
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E114I

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCerulean3-LaminB1-10

   Plasmid

   #55425

   Purpose

   Localization: Nuclear Envelope, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. FCK-ss-Prl-Arch-GFP

   Plasmid

   #22224

   Purpose

   Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   ss-Prl-Arch-GFP
   

   Use

   Lentiviral

   Tags

   GFP, Prl, and ss

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Jan. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-PTEN C124A

   Plasmid

   #50520

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C124A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

   Plasmid

   #202606

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-HA-Nrxn1α-LNS2

   Plasmid

   #128106

   Purpose

   Express HA tagged Nrxn1α-LNS2

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS2 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dmBACCS2VL-IRES-dOrai

   Plasmid

   #67629

   Purpose

   Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2VL-IRES-dOrai
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199368

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-ER-EmEGFP

    Plasmid

    #169000

    Purpose

    Express ER-EmEGFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
    

    Use

    Unspecified

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBItetDT12nGFP

    Plasmid

    #80420

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLPC-mCh-ACTB-P2A-EGFP

    Plasmid

    #165158

    Purpose

    Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    mCherry-ACTB-P2A-EGFP (ACTB Human)
    

    Use

    Retroviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pclbw-PAGFPomp25

    Plasmid

    #58427

    Purpose

    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    paGFP-omp25 (Synj2bp Mouse)
    

    Use

    Retroviral

    Tags

    PAGFP

    Expression

    Mammalian

    Mutation

    contains aa's 109-145 of mouse omp25

    Promoter

    cmv-bactin

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAG-eYFP-3x-miR708-5p-TS

    Plasmid

    #117381

    Purpose

    An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-mNRDc-E>A-V5

    Plasmid

    #51298

    Purpose

    expression of the inactive mutant of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc E247A (Nrd1 Mouse)
    

    Use

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    E247A, enzymatic inactive mutant

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-HA-Nrxn1α-LNS4

    Plasmid

    #128108

    Purpose

    Express HA tagged Nrxn1α-LNS4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS4 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-HA-Nrxn1α-LNS5

    Plasmid

    #128109

    Purpose

    Express HA tagged Nrxn1α-LNS5

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS5 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pST_AfrLEA3m (pBS0911)

    Plasmid

    #185285

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pST_PvLEA4_repeats_k3_26 (pBS0762)

    Plasmid

    #185244

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mOrange2-Clathrin-15

    Plasmid

    #57947

    Purpose

    Localization: Clathrin Vesicles, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pRSET-pr-mEos4b (mEosFP4b-V69T)

    Plasmid

    #99226

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXSIP-GPR35-FLAG-APEX

    Plasmid

    #204706

    Purpose

    expresses GPR35-FLAG-APEX in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Use

    Tags

    FLAG, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPA-fapO-RgTALsyn

    Plasmid

    #100946

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    GAP (Constitutive)

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEmerald-MyosinIIC-C-18

    Plasmid

    #54194

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits