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pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Publication

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pBPGAL4.2::VP16Uw

Plasmid

#26228

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::VP16 (GAL4 Budding Yeast)

Expression

Insect

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGADCg

Plasmid

#20161

Depositor

Peter Uetz

Publication

Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

Type

Empty backbone

Tags

Gal4 AD and SV40 NLS

Expression

Yeast

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Expression

Worm

Promoter

U6 promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMXs-mRFP1

Plasmid

#21315

Purpose

MoMLV-based Retroviral vector, expresses mRFP1

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

monomeric Red Fluorescence Protein 1

Use

Retroviral

Expression

Mammalian

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hsyn-GRAB_DA2m

Plasmid

#140553

Purpose

Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2m (medium affinity)

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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Flag-HA-USP46

Plasmid

#22584

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP46 (USP46 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_Floxed-STOP mCherry

Plasmid

#122963

Purpose

mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-Rab5A

Plasmid

#28043

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5A (RAB5A Human)

Tags

3xFlag

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBabe-GFP-IKBalpha-wt

Plasmid

#15263

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Myc-Foxo1 D256

Plasmid

#12145

Depositor

Domenico Accili

Publication

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Foxo1 (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Contains a deletion truncating the protein after …

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTSA-13

Plasmid

#17327

Depositor

Takeshi Sano

Publication

Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV HA hRB-wt

Plasmid

#58905

Purpose

mammalian expression of wild type human Rb

Depositor

Steven Dowdy

Publication

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Publication

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Promoter

c-fos

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLV-EBFP2-nuc

Plasmid

#36085

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-BRCC3

Plasmid

#22540

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BRCC3 (BRCC3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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