We narrowed to 18,568 results for: REV

1. pET21b-rrt-His8

   Plasmid

   #194088

   Purpose

   Expresses Ec86 reverse transcriptase in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 rrt
   

   Tags

   His8

   Promoter

   T7

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pILGFPH7

   Plasmid

   #83554

   Purpose

   Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   ADH2 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   ADH2

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p413TEF-SirT1

   Plasmid

   #51746

   Purpose

   overexpression of human SirT1 in S. cerevisiae, HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   SirT1 (SIRT1 Human)
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMJB1-RPS22B-Cl

   Plasmid

   #160431

   Purpose

   Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   RPS22B (RPS22B Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Removed introns, shuffled codons to change nucleo…

   Promoter

   GAL1 and S. cerevisiae GAL1

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEN41A-T38S38

   Plasmid

   #49524

   Purpose

   Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   constitutive TetR38
   

   Promoter

   Ptb38

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILGFP97

   Plasmid

   #83551

   Purpose

   Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CYC1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   CYC1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEasyG1_zeo

   Plasmid

   #184910

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p414GPD-3xFLAG-Nop4 WT

   Plasmid

   #169261

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 WT (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET151-Vps4(101-437)

   Plasmid

   #87735

   Purpose

   Bacterial expression of N-terminally truncated S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH131

    Plasmid

    #48260

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    GAL10/GAL1 bidirectional

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH353-SUP45wt

    Plasmid

    #29371

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Wild type gene including genomic sequence 1432 bp…

    Available Since

    May 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFP86

    Plasmid

    #83543

    Purpose

    Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL4A promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    RPL4A

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    SSA1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGS1011

    Plasmid

    #63783

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Strep-tag II followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Strep-tag II

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH104

    Plasmid

    #48262

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    

    Use

    Routine cloning vector

    Mutation

    From SK1 background. -343 to -1 relative to TEF1 …

    Promoter

    TEF1p

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET151-Vta1(280-330) S278C

    Plasmid

    #87739

    Purpose

    Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-277, mutated Ser 278 to Cys

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET151-Vps4(81-437)

    Plasmid

    #87734

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-80

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dom34(265-386)

    Plasmid

    #19102

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag and N tag

    Expression

    Bacterial

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only