Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 28,730 results for: his

Showing: 3361 - 3380 of 28730 results

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pRSETb 6xHis-Cystector (Cysteine biosensor)

Plasmid

#250652

Purpose

E. coli expression plasmid for 6xHisTag-Cystector (Cysteine biosensor)

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA W37V

Plasmid

#248802

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST14-His6-TEV-SpyCatcher003-cys

Plasmid

#249543

Purpose

Expresses SpyCatcher003 with a N-terminal 6x-His tag and a C-terminal cysteine in E.coli under the T7 promoter

Depositor

Kai Zinn

Article

Anaya et al bioRxiv 2025.07.09.663943

Insert

SpyCatcher003-cys

Tags

6x His and Cysteine

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2288 - pET28-mTagBFP-SRT-His6

Plasmid

#250250

Purpose

Recombinant production of sortase-tagged mTagBFP.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

mTagBFP

Tags

Sortase recognition site, 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mDE-Myc-His

Plasmid

#249180

Purpose

AAV mediated overexpression of mouse Sparcl1 a.a. 330-550 fragment

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mSLF-Myc-His

Plasmid

#249179

Purpose

AAV mediated overexpression of C-terminal fragment of mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

C-terminal fragment

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mHevin-Myc-His

Plasmid

#249178

Purpose

AAV mediated overexpression of full length mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-DE-Myc-His

Plasmid

#249176

Purpose

Overexpression of mouse Sparcl1 fragment a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and C55I

Plasmid

#248807

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and C55I

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and R337P

Plasmid

#248805

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and R337P

Plasmid

#248817

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 D364E-His

Plasmid

#246711

Purpose

Expression vector for secreted human GPR124 ECD D364E (RGD -> RGE), N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD D364E

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-GPR124 LRR-His

Plasmid

#246695

Purpose

Expression vector for secreted human GPR124 LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

LRR domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔGAIN-His

Plasmid

#246693

Purpose

Expression vector for secreted human GPR124 ECD lacking the GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 427-755

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔHRM-His

Plasmid

#246692

Purpose

Expression vector for secreted human GPR124 ECD lacking the HRM domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 349-426

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-GPR124 GAIN-His

Plasmid

#246694

Purpose

Expression vector for secreted human GPR124 GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

GAIN domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124 ΔLRR-His

Plasmid

#246691

Purpose

Expression vector for secreted human GPR124 ECD lacking LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 34-247

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and C55M

Plasmid

#248819

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and C55M

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and P167I

Plasmid

#248816

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and P167I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and T57I

Plasmid

#248806

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and T57I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3361 - 3380 of 28730 results

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