We narrowed to 6,705 results for: hwa

1. pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

   Plasmid

   #52720

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 328–447

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK-Venus-TmiR-G13-G12

   Plasmid

   #25742

   Purpose

   Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 13 and 12 miR-shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Venus

   Expression

   Mammalian

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-UAP56 MUT

   Plasmid

   #110124

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KSLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Opto-GPR173

   Plasmid

   #106058

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR173 (GPR173 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-CHMP4C (216-233) (pGEX2T(TEV))

   Plasmid

   #80635

   Purpose

   Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   CHMP4C (CHMP4C Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 216-233

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-F3UXG6

   Plasmid

   #103108

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Opto-GPR31

   Plasmid

   #106014

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR31 (GPR31 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-C7TEQ6

   Plasmid

   #103092

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

   Plasmid

   #16753

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-deltaCTER (DVL3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   delta CTER

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-mDIXDC1 WT

    Plasmid

    #61216

    Purpose

    Entry clone encoding long isoform of mouse DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    DIXDC1 (Dixdc1 Mouse)
    

    Use

    Donr clone (gateway)

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Opto-GPR25

    Plasmid

    #106011

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR25 (GPR25 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-TPI_WT-4MS2-xrRNA-4H

    Plasmid

    #108371

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1161

    Plasmid

    #111097

    Purpose

    Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCin4-3xFLAG-MKL1 L272A

    Plasmid

    #19850

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 L272A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 272 to Alanine

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pCR1110

    Plasmid

    #111096

    Purpose

    Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    BLM gRNA (BLM Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLXSN-H-Ras_V12-S35

    Plasmid

    #39517

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 S35 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V and T35S

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCin4-3xFLAG-MKL1 D444-630

    Plasmid

    #19840

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-630 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-630

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid

    #52725

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed cysteines 12 and 15 to alanines

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Opto-GPR21

    Plasmid

    #106009

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR21 (GPR21 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Opto-GPR1

    Plasmid

    #105999

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR1 (GPR1 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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