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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 321 - 340 of 4550 results

  2. pMBEC8

    Plasmid
    #187599
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187599-plasmid-map-sequence-id-367572

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  5. BFP-KASH2

    Plasmid
    #187019
    Purpose
    Inducible disruption of LINC Complex
    Depositor
    Jan Lammerding
    Insert
    KASH2
    Tags
    tagBFP
    Expression
    Mammalian
    Available Since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    187019-plasmid-map-sequence-id-367150

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  6. BFP-KASH2ext

    Plasmid
    #187020
    Purpose
    Control for inducible disruption of LINC Complex
    Depositor
    Jan Lammerding
    Insert
    KASH2ext
    Tags
    tagBFP
    Expression
    Mammalian
    Available Since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    187020-plasmid-map-sequence-id-367312

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  10. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  11. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid
    #187954
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187954-plasmid-map-sequence-id-373498

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Showing: 321 - 340 of 4550 results