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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 974 results for: Gatc

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Showing: 321 - 340 of 974 results

  3. pCRISP_IS

    Plasmid
    #120425
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    constitutive
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120425-plasmid-map-sequence-id-240993

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  6. pU1/Sm/3' Box_(GLuc)_INT

    Plasmid
    #68438
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U1
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68438-plasmid-map-sequence-id-125562

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  8. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid
    #68432
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68432-plasmid-map-sequence-id-125560

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  9. 1161F

    Plasmid
    #183138
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183138-plasmid-map-sequence-id-373781

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  13. pGEX2TK-YAP-WW1-RRK

    Plasmid
    #104306
    Purpose
    bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated
    Depositor
    Marius Sudol
    Insert
    YAP WW1 domain (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…
    Promoter
    tac
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104306-plasmid-map-sequence-id-201230

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  17. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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Showing: 321 - 340 of 974 results