We narrowed to 13,934 results for: TIM;

1. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E

   Plasmid

   #163998

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1427E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R

   Plasmid

   #163997

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1414R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R

   Plasmid

   #163996

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1411R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R

   Plasmid

   #163994

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1397R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

   Plasmid

   #163993

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1392E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

   Plasmid

   #163992

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1386E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

   Plasmid

   #198035

   Purpose

   Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   SINTBAD (TBKBP1 Human)
   

   Use

   Tags

   EGFP and GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 10xUAS-wgSS-HA-V5-LaccID-CD2 (fly optimized, repeat fixed)

   Plasmid

   #234050

   Purpose

   expresses LaccID on the surface of fly neurons

   Depositor

   Alice Ting

   Article

   Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

   Insert

   LaccID-CD2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

   Plasmid

   #139444

   Purpose

   Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   sequence codon-optimized

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

    Plasmid

    #166834

    Purpose

    Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

    Plasmid

    #199566

    Purpose

    Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Andrea Cimarelli, Yuxin Song

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210734

    Purpose

    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Frodo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210735

    Purpose

    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Gollum sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210736

    Purpose

    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Merry sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

    Plasmid

    #39299

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. human EB1 fused to monomeric RFP

    Plasmid

    #39323

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Inserts

    EB1 (MAPRE1 Human)
    mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUW H2B-mTagBFP2-P2A-PuroR

    Plasmid

    #239552

    Purpose

    Expression and nuclear localization of mTagBFP2

    Depositor

    Joel Blanchard, Tim Ahfeldt

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbC

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Actin-mRFP-PAGFP

    Plasmid

    #62382

    Purpose

    Expresses beta actin with both a PAGFP and mRFP tag.

    Depositor

    Tim Mitchison, Guillaume Charras

    Article

    Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

    Insert

    beta actin (ACTB Human)
    

    Use

    Tags

    PAGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Use

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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