We narrowed to 19,472 results for: TIM;

1. Lck-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129627

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Lck-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-STIM1-NN_ST-SBP-mCherry

   Plasmid

   #65263

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3xnls-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129626

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-TNT-CFP-STIM1-C227W

   Plasmid

   #68403

   Purpose

   Expresses a STIM1 gain-of-function mutant in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

   Insert

   CFP-STIM1 (STIM1 Human)
   

   Use

   Tags

   Cyan Fluorescent Protein (CFP)

   Expression

   Mammalian

   Mutation

   Changed Cysteine 227 to Tryptophan; CFP was inser…

   Promoter

   CMV

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mNeongreen-pGBD(PKN1 codon optimized)

   Plasmid

   #129634

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   RhoA binding doamin of PKN1(30 - 100) codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM-60-HsAGO2-448-859-optim_K

   Plasmid

   #146729

   Purpose

   Bacterial Expression of HsAGO2_448-859-optim

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2_448-859-optim (AGO2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR-ZmIAA29 (yeast codon optimized)

   Plasmid

   #188441

   Purpose

   Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Repressor IAA29 (yeast codon optimized)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-732pUC19-tNGFR-P2A-STIM1

   Plasmid

   #186119

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STIM1 (STIM1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag‐hTim1

    Plasmid

    #49207

    Purpose

    expresses Flag-tagged human Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (HAVCR1 Human)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    Tim1 lacks its own signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

12. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. STIMATE-mCherry

    Plasmid

    #70676

    Purpose

    Expresses STIMATE or TMEM110 in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (TMEM110 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEGFPN1‐mTim1

    Plasmid

    #49215

    Purpose

    expresses EGFP tagged mouse Tim1

    Depositor

    Lawrence Kane

    Insert

    Tim1 (Havcr1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5M-TIMP3

    Plasmid

    #31715

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TIMP3 (TIMP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag‐mTim1 (Bl/6)

    Plasmid

    #49205

    Purpose

    expresses Flag tagged mouse Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (BL/6 allele) (Havcr1 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    Tim1 lacks its own signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

19. STIMATE(1-240)

    Plasmid

    #71280

    Purpose

    Expresses STIMATE or TMEM110 (1-240) in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (1-240) (TMEM110 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Stim1-CaRQ

    Plasmid

    #34560

    Depositor

    Kevin Truong

    Article

    Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

    Insert

    Stim1-CaRQ (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L in RhoA

    Promoter

    CMV, T7

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only