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pPB-cT3G-cERP2-RUNX1

Plasmid

#192928

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHDR-FOXL2-T2A-tdTomato-PuroTK

Plasmid

#192892

Purpose

HDR donor for inserting FOXL2-T2A-tdTomato reporter

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2-tdTomato (FOXL2 Human)

Use

CRISPR

Mutation

T2A-tdTomato fluorescent reporter

Promoter

PGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-EGR1

Plasmid

#192913

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

EGR1 (EGR1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOS

Plasmid

#192924

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOS

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOS (FOS Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-CEBPD

Plasmid

#192897

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of CEBPD

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

CEBPD (CEBPD Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOPX

Plasmid

#192923

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

HOPX (HOPX Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-2A-SOX17-GR-IH

Plasmid

#165079

Purpose

Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Inserts

rtTA-M2
SOX17 (SOX17 Human)

Use

Piggybac transposition

Tags

Glucocorticoid Receptor

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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TV-hPITX3-Reverse

Plasmid

#22208

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3-eGFP-loxP-PGK-Puro-loxP (PITX3, PGK1 Human)

Use

Zfn donor

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KLF6

Plasmid

#192909

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

KLF6 (KLF6 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KLF4

Plasmid

#192908

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

KLF4 (KLF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-JUN

Plasmid

#192895

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of JUN

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

JUN (JUN Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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