We narrowed to 416 results for: ipsc

1. pGS_101

   Plasmid

   #160541

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGS_107

   Plasmid

   #160650

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGS_129

   Plasmid

   #160652

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-28a(+)-FGF2-K128N

   Plasmid

   #120284

   Purpose

   Produce human recombinant FGF2 K128N in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-32a(+)-TGFB1

   Plasmid

   #120361

   Purpose

   Produce human recombinant TGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β1 (TGFB1 Synthetic, Human)
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERP2-FOS

   Plasmid

   #192924

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOS

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOS (FOS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-cT3G-cERP2-HOPX

   Plasmid

   #192923

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOPX (HOPX Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPBCAG-rtTAM2-2A-SOX17-GR-IH

   Plasmid

   #165079

   Purpose

   Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Inserts

   rtTA-M2
   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposition

   Tags

   Glucocorticoid Receptor

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-cT3G-cERP2-KLF6

    Plasmid

    #192909

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF6 (KLF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TV-hPITX3-Reverse

    Plasmid

    #22208

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3-eGFP-loxP-PGK-Puro-loxP (PITX3, PGK1 Human)
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB-cT3G-cERP2-KLF4

    Plasmid

    #192908

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-cT3G-cERP2-JUN

    Plasmid

    #192895

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of JUN

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    JUN (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-TetOminiCMV-KSM

    Plasmid

    #136553

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4-IRES-Sox2-E2A-cMyc (Klf4, Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-TetOminiCMV-OSM

    Plasmid

    #136555

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1a-PEmax-P2A-GFP

    Plasmid

    #202000

    Purpose

    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

    Depositor

    Daniel Geschwind

    Insert

    PEmax-P2A-GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCE-KdGl

    Plasmid

    #154880

    Purpose

    Episomally expresses KDM4D and GLIS1 in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

    Insert

    KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge