We narrowed to 986 results for: transfection

1. pOO2-GW

   Plasmid

   #135097

   Purpose

   Gateway vector for expression of proteins in Xenopus oocytes

   Depositor

   Wolf Frommer

   Article

   Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

   Type

   Empty backbone

   Use

   Bacterial expression vector for generating sp6 tr…

   Promoter

   SP6

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-mios in pRK5

   Plasmid

   #154212

   Purpose

   N-terminal HA-tagged Mios cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   Mios
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   cDNA sequence has been codon optimized using huma…

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. RDX-mCherry

   Plasmid

   #214476

   Purpose

   Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   RDX (RDX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Sequence matches cDNA clone MGC:48283 IMAGE:52844…

   Promoter

   CMV

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SEH1L in pRK5

   Plasmid

   #154216

   Purpose

   Untagged SEH1L cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   SEH1L (SEH1L Human)
   

   Tags

   NONE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MPRA Empty Vector

   Plasmid

   #204865

   Purpose

   Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

   Depositor

   Philipp Maass

   Article

   Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-DDK

   Plasmid

   #137764

   Purpose

   Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GFP
   

   Tags

   DDK

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTarget TMEM97-P2A-mCardinal

   Plasmid

   #157747

   Purpose

   Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xMx PylT C41CA_GFP 150TAG

    Plasmid

    #140017

    Purpose

    Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIhh742bp-Luc

    Plasmid

    #53605

    Purpose

    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIhh1.3K-Luc

    Plasmid

    #53604

    Purpose

    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDOE11 mas:mTq2 transient

    Plasmid

    #124248

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIhh715bp-Luc

    Plasmid

    #53606

    Purpose

    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBOB-3xFlag-hGpr75-p.Gln234*

    Plasmid

    #236748

    Purpose

    Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    GPR75 (GPR75 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Gln234 to STOP codon, CAA to TAA

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBOB-3xFlag-hGpr75-p.Ala110fs

    Plasmid

    #236747

    Purpose

    Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    GPR75 (GPR75 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Ala110fs, deletion of GCCAGTA

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. FHpHG_mTent5a

    Plasmid

    #221439

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5a (Fam46a Mouse)
    

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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