We narrowed to 1,038 results for: transfection
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Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
TagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_GFP 150TAG
Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-mios in pRK5
Plasmid#154212PurposeN-terminal HA-tagged Mios cDNA expression vector for transfectionsDepositorInsertMios
TagsHA tagExpressionMammalianMutationcDNA sequence has been codon optimized using huma…PromoterCMVAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
shh10
Plasmid#64867PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.DepositorInsertshh10 cap
UseAAVMutationI319V, N451D, D532NAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTarget TMEM97-P2A-mCardinal
Plasmid#157747PurposeExpress in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.DepositorInsertTMEM97 (TMEM97 Human)
ExpressionMammalianAvailable SinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MPRA Empty Vector
Plasmid#204865PurposeVector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.DepositorTypeEmpty backboneExpressionBacterial and MammalianAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5a
Plasmid#221439PurposeTransfection into the cells for the reporter or qPCR assay.DepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
tdTomato/pTREX-b
Plasmid#68709PurposeExpresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.DepositorInserttdTomato
UseExpression of tdtomato red fluorescence protein i…Available SinceOct. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDOE11 Lat52:mCherry transient
Plasmid#124249PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Fosprom
Plasmid#188113PurposeExpresses luciferase under control of Fos promoter in transfected cellsDepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GLUT5 in pRK5
Plasmid#154205PurposeN-terminal HA-tagged GLUT5 cDNA expression vector for transfectionsDepositorAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOO2-GW
Plasmid#135097PurposeGateway vector for expression of proteins in Xenopus oocytesDepositorTypeEmpty backboneUseBacterial expression vector for generating sp6 tr…PromoterSP6Available SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TVMV-TEV-HRV-3C Proteases-mCherry
Plasmid#243798PurposeEncodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV, TEV and HRV-3C proteases
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
SEH1L in pRK5
Plasmid#154216PurposeUntagged SEH1L cDNA expression vector for transfectionsDepositorAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only