We narrowed to 30,796 results for: Ide

1. pcDNA3-FLAG-Synaptojanin 1-170

   Plasmid

   #22292

   Depositor

   Pietro De Camilli

   Article

   Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

   Insert

   Synaptojanin 1_170 (SYNJ1 Human)
   

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   K334R compared to Genbank ID NM_003895

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

   Plasmid

   #208046

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Plxnb2(L)-Fc-His

   Plasmid

   #72128

   Purpose

   Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb2 (Plxnb2 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATP1A1_plasmid_donor_RD

   Plasmid

   #86551

   Purpose

   Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 (ATP1A1 Human)
   

   Use

   CRISPR and TALEN; Co-selection via hdr using ouab…

   Expression

   Mammalian

   Mutation

   Q118R, N129D

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS34.EF1α<GFP-2A-Cas9-RC

   Plasmid

   #207404

   Purpose

   Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Cas9-RC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP-2A

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1cam-Fc-His

   Plasmid

   #72083

   Purpose

   Expresses the extracellular region of the L1CAM protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   L1cam (L1cam Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cntn1-Fc-His

   Plasmid

   #72065

   Purpose

   Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Cntn1 (Cntn1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Jam2-Fc-His

   Plasmid

   #72079

   Purpose

   Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Jam2 (Jam2 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid

    #208047

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Mutation

    Cys>Ala mutations in conserved RING domain

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Plxnb2(S)-Fc-His

    Plasmid

    #72129

    Purpose

    Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid

    #180644

    Purpose

    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    Residues 833-1050

    Promoter

    pCAG

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lrrc4-Fc-His

    Plasmid

    #72084

    Purpose

    Expresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4 (Lrrc4 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cntn2-Fc-His

    Plasmid

    #72066

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Jam1-Fc-His

    Plasmid

    #72078

    Purpose

    Expresses the extracellular region of the JAM-1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam1 (F11r Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cntn1-AP-His

    Plasmid

    #71939

    Purpose

    Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn1 (Cntn1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Jam1-AP-His

    Plasmid

    #71952

    Purpose

    Expresses the extracellular region of the JAM-1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam1 (F11r Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cntn2-AP-His

    Plasmid

    #71940

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cntn5.1-AP-His

    Plasmid

    #71943

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cntn5.1-Fc-His

    Plasmid

    #72069

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge