We narrowed to 22,823 results for: Sis

1. pRetroX-Tight-MCS_PGK-GpNLuc

   Plasmid

   #70185

   Purpose

   Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKan-AID*-9myc

   Plasmid

   #99522

   Purpose

   C-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-TetO-PM

   Plasmid

   #197387

   Purpose

   To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral; Teto

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER-retro-puro-tetO-CTR1-shRNA

   Plasmid

   #53180

   Purpose

   Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHis-AID*-9myc

   Plasmid

   #99524

   Purpose

   C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWJB9

    Plasmid

    #126650

    Purpose

    Expresses AdrA from a modified pLAFR3

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKH3-p190

    Plasmid

    #15547

    Purpose

    Expression of HA-tagged p190 RhoGAP

    Depositor

    Ian Macara

    Article

    Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

    Insert

    p190 GTPase activating protein (Arhgap35 Rat)
    

    Use

    Tags

    Triple HA1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pHyg-AID*-9myc

    Plasmid

    #99518

    Purpose

    C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES-Ap2a2-FKBP

    Plasmid

    #46944

    Purpose

    Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap2a2-FKBP
    

    Use

    Tags

    Mainly mouse Ap2a2 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMB1-A-TelR

    Plasmid

    #204979

    Purpose

    Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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