We narrowed to 18,824 results for: nls
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Plasmid#69223PurposeExpresses R1335K mutant SpCas9 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationArginine 1335 is changed to LysineAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-miRFP670nano-miRFP670
Plasmid#216131PurposeLentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.DepositorInsertEF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
UseLentiviralExpressionMammalianAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
Plasmid#220613PurposeCre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.DepositorInsertFLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianPromoterEF1aAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet
Plasmid#220608PurposeNeuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianPromoterhSynAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet
Plasmid#220614PurposeCre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mA1-BE3
Plasmid#113421PurposeExpresses mA1-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmA1-BE3 (Apobec1 S. pyogenes and Bacteriophage PBS2, Mouse)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBLO1806_Cas9_2xNLS_human
Plasmid#74493PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNADepositorInsertCas9
Tagst2a mCherryExpressionMammalianAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry
Plasmid#230043PurposePartial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
UseCre/Lox; Zebrafish expressionPromoterfabp10aAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159867PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB_UCOE-CAG-2xMeCP2NLS-Cas9-NpNLS-FLAG-TagBFP-2xSV40NLS-rBGpA_chs4_EF1a-Blast-rBGpA
Plasmid#254793PurposeConstituitve expression of spCas9 with optimized nuclear localization for iPSC-derived neurons via transposase integrationDepositorInsert2xMeCP2NLS-Cas9-NpNLS-FLAG-TagBFP-2xSV40NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceSept. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-CaM
Plasmid#183401PurposeExpresses nuclear-localized LgBiT-CaMDepositorInsertLgBiT-CaM
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-KRAB
Plasmid#126589PurposeExpresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backboneDepositorInsert2XMCP-KRAB
UseLentiviralTagsNLS-HAExpressionMammalianPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:PYL1:nls:VPR
Plasmid#135988PurposeExpresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertPYL1 domain fused to the VPR activation domain
TagsHisExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLBS.CAG/NLS-mScarlet
Plasmid#129336Purposenucleus marker, lentivirusDepositorInsertmScarlet (Addgene 85042)
UseLentiviralAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
TagsEGFP and FLAG-NLSExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NLS-LucDM-GFP
Plasmid#127796PurposeThe expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdestabilized mutant (DM) Luciferase
TagsEGFP and FLAG-NLSExpressionMammalianMutationR188Q, R261Q in Firefly Luciferase insertPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP
Plasmid#230075PurposePartial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
UseCre/Lox; Zebrafish expressionPromoterfabp10aAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
BC1177-pHR-SFFV-GFP(1-10)-NLS
Plasmid#164039PurposeExpression of GFP1-10 in mammalian cellsDepositorInsertGFP(1-10)
UseLentiviralExpressionMammalianPromoterSFFVAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP453 pEf1a-NLS-aGCN4-mCherry
Plasmid#100945PurposemCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, mCherry
Tags3xTy1ExpressionMammalianAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only