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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 3401 - 3420 of 6562 results

  3. mKikGR-PCNA-19-SV40NLS-4

    Plasmid
    #57328
    Purpose
    Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mKikGR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100087-plasmid-map-sequence-id-197556

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  8. mPA-GFP-PCNA-19-SV40NLS-4

    Plasmid
    #57154
    Purpose
    Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. RPL25NLS-GFP-PRA (102)

    Plasmid
    #24041
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP and PRA
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Available since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  12. SpCas9-ABE8e(V106W)

    Plasmid
    #198547
    Purpose
    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198547-plasmid-map-sequence-id-388882

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  13. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid
    #80769
    Purpose
    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).
    Depositor
    Kazuhisa Nakayama
    Publication
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFT88 gRNA#1 (IFT88 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80769-plasmid-map-sequence-id-171039

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  14. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid
    #120506
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Publication
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC H84NLS (MYC Human)
    Use
    Lentiviral
    Mutation
    Deletion of nuclear localization signal sequence
    Promoter
    EF1a
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120506-plasmid-map-sequence-id-234926

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  20. pBA2675

    Plasmid
    #189997
    Purpose
    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
    Depositor
    Bungo Akiyoshi
    Publication
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    NLS
    Mutation
    N-terminal 600 bp of FBP75
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189997-plasmid-map-sequence-id-371586

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Showing: 3401 - 3420 of 6562 results