We narrowed to 5,806 results for: pcas

1. pEPOR2KN0001

   Plasmid

   #117576

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR0SP0015

   Plasmid

   #117525

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (no stop codon)
   

   Use

   CRISPR

   Mutation

   K855A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR0SP0001

   Plasmid

   #117523

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-DE (no stop codon)
   

   Use

   CRISPR

   Mutation

   D1135E

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR2KN0049

   Plasmid

   #117616

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEditC

   Plasmid

   #207532

   Purpose

   Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEditA-RY

   Plasmid

   #207533

   Purpose

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEditC-RY

   Plasmid

   #207534

   Purpose

   Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10530

    Plasmid

    #239266

    Purpose

    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    NES-dPspCas13b-EGFP-ABI
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag tag and V5 tag

    Expression

    Mammalian

    Mutation

    H133A and H1058A in PspCas13b

    Promoter

    EF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-BsaIx2-DD-Cas9

    Plasmid

    #75380

    Purpose

    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SiC-V1-Scr

    Plasmid

    #133042

    Purpose

    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL063

    Plasmid

    #124895

    Purpose

    For genome editing in plant by SpCas9 variant(SpCas9-NG)

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMP416

    Plasmid

    #134996

    Purpose

    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    

    Use

    Synthetic Biology

    Tags

    3xFLAG, E coli Dam(N132A), and dCas9

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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