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pET11a_3xFlag-nsp14 (SARS-CoV-2)

Plasmid

#169159

Purpose

To express SARS-CoV-2 nsp14 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)

Tags

3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

Plasmid

#197504

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-ABE8e-NRCH-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-PuroR [M1G]

Plasmid

#171809

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-BlastR [M1G]

Plasmid

#171805

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

Plasmid

#122981

Purpose

AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Rat, Synthetic)

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

Plasmid

#170197

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

Promoter

CMV51

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

Plasmid

#170198

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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Spike Display_Part 2 Spacer

Plasmid

#172730

Purpose

Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET11a_3xFlag-nsp10 (SARS-CoV-2)

Plasmid

#169157

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab SARS-CoV-2, Synthetic)

Tags

3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

Plasmid

#197505

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

Promoter

CMV and T7

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

Plasmid

#170188

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

Promoter

CMV51

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Gamma)

Plasmid

#241115

Purpose

Expresses SARS-CoV-2 spike (Gamma) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Gamma) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L18F,T20N,P26S,D138Y,R190S,K417T,E484K,N501Y,D614…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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