We narrowed to 9,799 results for: otos

1. pAAV-Ef1a-FAS-GCaMP6f-WPRE

   Plasmid

   #141237

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   Human elongation factor-1 alpha (EF-1 alpha)

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-BOS-LGP2-Myc

   Plasmid

   #215874

   Purpose

   LGP2-Myc expression vector for overexpression

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

   Insert

   LGP2 (DHX58 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GfaABC1D-Crym-BioID2-BioID2

   Plasmid

   #200070

   Purpose

   To study u-crystallin protein interactors

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   GfaABC1D-Crym-BioID2-BioID2
   

   Use

   AAV and Mouse Targeting

   Tags

   HA-tag

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMx/ATP11C-b(E184Q)-HA

   Plasmid

   #209264

   Purpose

   Mammalian expression of ATP11C isoform b mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform b (ATP11C Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   E184Q

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG/ATP11C-c-HA

   Plasmid

   #209222

   Purpose

   Mammalian expression of ATP11C isoform c

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform c (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG/ATP11C-b-HA

   Plasmid

   #209221

   Purpose

   Mammalian expression of ATP11C isoform b

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform b (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-ST-P2A-pSC-CAAX

   Plasmid

   #207637

   Purpose

   A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXS-ATPIF1 E55A

   Plasmid

   #204704

   Purpose

   expresses ATPIF1 E55A in mammalian cells

   Depositor

   William Kaelin

   Article

   Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

   Insert

   ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E55A

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Shim_ProRS_TP_GFP_pK7FWG2

    Plasmid

    #202651

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic ProRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Shim_ThrRS_TP_GFP_pK7FWG2

    Plasmid

    #202652

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic ThrRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Shim_TrpRS_TP_GFP_pK7FWG2

    Plasmid

    #202653

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic TrpRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Shim_AspRS_TP_GFP_pK7FWG2

    Plasmid

    #202647

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic AspRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Shim_GlyRS_TP_GFP_pK7FWG2

    Plasmid

    #202648

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic GlyRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Shim_AsnRS_TP_GFP_pK7FWG2

    Plasmid

    #202649

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic AsnRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Shim_MetRS_TP_GFP_pK7FWG2

    Plasmid

    #202650

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic MetRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pUAST ɑSynWT-EGFP

    Plasmid

    #201249

    Purpose

    allows the expression of the insert in Drosophila melanogaster

    Depositor

    Pedro Domingos

    Article

    Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

    Insert

    ɑ-Synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1-zeo E321K ORF29

    Plasmid

    #200025

    Purpose

    Lentiviral vector for expression of KSHV ORF29 with E321K mutation

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF29
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glutamic acid 321 changed to lysine

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-zeo G226A E321K ORF29

    Plasmid

    #200026

    Purpose

    Lentiviral vector for expression of KSHV ORF29 with G226A and E321K mutation

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF29
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 226 changed to alanine, glutamic acid 321…

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-zeo D476A ORF29

    Plasmid

    #200027

    Purpose

    Lentiviral vector for expression of KSHV ORF29 with D476A mutation

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF29
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Aspartic acid 476 changed to Alanine

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3.1_B1ChR2_TS_mScarlet_ER

    Plasmid

    #195191

    Purpose

    Highly K+ selective channelrhodopsin

    Depositor

    Peter Hegemann, Johannes Vierock

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Bilabrum sp. K+ selective channelrhodopsin 2
    

    Use

    Tags

    ER-export sequence, Kir2.1 trafficking motif, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV_CaMKIIa_SERT_mCherry

    Plasmid

    #198736

    Purpose

    Serotonin transporter with mCherry fluorescent protein driven by CaMKIIa promoter on a AAV vector

    Depositor

    Lin Tian

    Article

    Vargas et al Science. 2023 Feb 17;379(6633):700-706.

    Insert

    Serotonin transporter and mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCas4_1_Δ2_VA

    Plasmid

    #186439

    Purpose

    Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCasΔ4_1_2_VA

    Plasmid

    #186440

    Purpose

    Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCas-4/1Δ2_VB

    Plasmid

    #186443

    Purpose

    Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCas12(PI)

    Plasmid

    #186448

    Purpose

    Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K613A, K617A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCas12b

    Plasmid

    #186449

    Purpose

    Encodes Cas12b from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCas12b(PI)

    Plasmid

    #186451

    Purpose

    Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R122A, G143P

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge