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pR562

Plasmid

#207886

Purpose

Creation of Level 0 entry clones

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

amilCP

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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OA984

Plasmid

#120363

Purpose

Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

Depositor

Omar Akbari

Publication

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 single chain antibody

Use

Unspecified

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEf1a-hifiDn29-dCas9-P2A-GFP

Plasmid

#247158

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Publication

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Publication

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-ExRai-AktAR2

Plasmid

#184051

Purpose

Golgi-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Publication

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Golgi-ExRai-AktAR2

Tags

N-terminal 35 amino acids from human endothelial …

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-Venus

Plasmid

#106076

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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RBD#3L

Plasmid

#193020

Purpose

hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Publication

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Tags

hnRNP A1 RRM2

Expression

Bacterial

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAL-rfp_T

Plasmid

#74075

Purpose

pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA rfp (T)

Use

Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pKK01

Plasmid

#64744

Purpose

Yeast expression plasmid: phaA, phaB, phaC

Depositor

Jens Nielsen

Publication

Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

Insert

phaA, phaB, phaC

Expression

Yeast

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Publication

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Publication

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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RFP-KIF1B beta-FL

Plasmid

#79111

Purpose

Mammalian expression of RFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Publication

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

RFP-KIF1B beta full length (KIF1B Human)

Tags

RFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pGFP-PTEN C124A

Plasmid

#50520

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Publication

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

C124A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
gCH128 (non-targeting crRNA)

Plasmid

#217339

Purpose

Non-targeting crRNA

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crNT-3

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

Plasmid

#61256

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Publication

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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