We narrowed to 9,251 results for: CAG

1. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1-a

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229792

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-FLEX-EGFP-mir30(Scn9a)

   Plasmid

   #79672

   Purpose

   Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP (Scn9a jellyfish, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX-SpiCee-NES

   Plasmid

   #140901

   Purpose

   mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NES
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-Neo-Mito-DsRed

   Plasmid

   #210328

   Purpose

   Overexpression of Mito-DsRed

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   Mito-DsRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIG2-Flag-mNuak1 WT

   Plasmid

   #168507

   Purpose

   For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   Nuak1 (Nuak1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid

    #183609

    Purpose

    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    Neo
    mKate2-3xNLS
    

    Use

    Mouse Targeting

    Tags

    3xNLS

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCX-Lyn-SpiCee

    Plasmid

    #140837

    Purpose

    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    Lyn-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-GfaABC1D-iCreV-4x6T

    Plasmid

    #196412

    Purpose

    Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    iCreV
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-FLEX-FLIM-AKART391A

    Plasmid

    #60446

    Purpose

    Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKART391A
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 391 to Ananine

    Promoter

    CAG

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

    Plasmid

    #162486

    Purpose

    For generating transgenic mice expressing ERK biosensor and RGECO1

    Depositor

    Michiyuki Matsuda

    Article

    Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

    Insert

    ERK biosensor based on FRET, and RGECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCellFree_KA1 ZIKV E

    Plasmid

    #145341

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCellFree_KA1_ZIKV NS5

    Plasmid

    #145348

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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