We narrowed to 2,346 results for: puc19

1. pC016 - LwCas13a guide expression backbone with U6 promoter

   Plasmid

   #91906

   Purpose

   Backbone for cloning LwCas13a guides under U6 promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Has Service

   Cloning Grade DNA

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

   Plasmid

   #68375

   Purpose

   Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and TALEN; Zfn

   Tags

   3xFLAG_Twin_Strep

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK1

   Available since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-AAVS1-Ef1a-Puro-moxGFP

   Plasmid

   #227273

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mStayGold-TJP1

   Plasmid

   #227300

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-AAVS1-Ef1a-Puro-mScarlet

   Plasmid

   #227274

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL703

   Plasmid

   #140373

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-2BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

   Plasmid

   #193049

   Purpose

   Encodes G-418 resistance

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   prsp-27::NeoR::unc-54 3’ UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   prsp-27

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-AAVS1-PGK-Puro-moxGFP

   Plasmid

   #227275

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS133

   Plasmid

   #211504

   Purpose

   AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   mTagBF2
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT1420

    Plasmid

    #228316

    Purpose

    Homoserine lactone (HSL)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Homoserine lactone (HSL)-regulatable synthetic pr…

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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